Methods of integrating target genes into the B. subtilis genome: (A)

Genome Editing Methods for Bacillus subtilis

Qi Guo's research works Jiangsu University, Zhenjiang and other

MiniBacillus PG10 as a Convenient and Effective Production Host

Integrative Plasmid - an overview

Schematic overview of the “All-In-One” genome integration method. (A)

Methods of integrating target genes into the B. subtilis genome: (A)

PDF] Integration of stable extracellular DNA released from Escherichia coli into the Bacillus subtilis genome vector by culture mix method

Barriers to simultaneous multilocus integration in Bacillus subtilis tumble down: development of a straightforward screening method for the colorimetric detection of one-step multiple gene insertion using the CRISPR-Cas9 system

DNA assembly by the ''domino method'' in the BGM vector. The figure

Scheme of the targeted mprB.amy-cassette integration into the JE852

Construction and application of an efficient dual-base editing platform for Bacillus subtilis evolution employing programmable base conversion - Chemical Science (RSC Publishing) DOI:10.1039/D2SC05824C

Bacillus integrative plasmid system combining a synthetic gene circuit for efficient genetic modifications of undomesticated Bacillus strains, Microbial Cell Factories

Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli

Part:BBa K3664024

Bottom-up genome assembly using the Bacillus subtilis genome vector